PCR success rates at any single locus ranged from 92.6 to 98.8% (fresh-processed) and 75.5 to 79.6% (mailed). Genotypes were obtained from 96.1% (1, 865 genotypes from 1,941 assays) of fresh-processed biosamples and 77.6% (681 genotypes from 878 assays) of mailed biosamples. A PCR success rate of 90.3% (2,546 genotypes from 2,819 assays) was achieved. We were able to obtain at least one genotype from 99.7% (645 of 647) of fresh-processed and 97.4% (330 of 339) of mailed biosamples. Six hundred forty-seven of these swabs were processed immediately after collection and 348 were received by mail. From January 1995 to December 1997, we processed 995 buccal swabs for use in polymerase chain reaction (PCR)-based genotype assays in the context of ongoing molecular epidemiologic studies. Buccal (cheek) swabs have been proposed as a means of achieving these goals, but there is little information about the practical application of this approach. Studies in molecular and genetic epidemiology require a high-throughput, low cost, and reliable means of genomic DNA collection. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications. The optimal DNA isolation could be analyzed by PCR technique. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The DNA was then isolated using buffer extraction method. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. Therefore, a study was carried out to look for an alternative of DNA isolation. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms.
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